Cyanine dye labeling reagents—carboxymethylindocyanine succinimidyl esters

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling.

Our objective was to study the properties of the carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) and the methodology of cell labeling using CFDA-SE fluorescent dye. First, we analyzed the kinetics of CFDA-SE fluorescent dye intensity over time. Second, we determined the optimal concentration of CFDA-SE fluorescent dye for cell labeling. Third, we tested the toxicity of CFDA-SE fluores...

متن کامل

Cyanine dye dUTP analogs for enzymatic labeling of DNA probes.

Fluorescence in situ hybridization (FISH) has become and indispensable tool in a variety of areas of research and clinical diagnostics. Many applications demand an approach for simultaneous detection of multiple target sequences that is rapid and simple, yet sensitive. In this work, we describe the synthesis of two new cyanine dye-labeled dUTP analogs, Cy3-dUTP and Cy5-dUTP. They are efficient ...

متن کامل

Rigid cyanine dye nucleic acid labels.

Cyanine dyes attached to DNA via a rigid linker show useful fluorescence and FRET properties without altering the stability of duplex DNA.

متن کامل

Specific labeling of polypeptides at amino-terminal cysteine residues using Cy5-benzyl thioester.

Even for moderately sized proteins, the multiple occurrence of cysteine and lysine residues often prevents the specific labeling of polypeptides with a single probe. To increase specificity, a method was developed to convert the commonly available succinimidyl esters of fluorescent dyes into benzyl thioesters via trimethyl aluminum-activated benzyl mercaptan. The thioester can then be reacted v...

متن کامل

Abnormal fluorescence behavior of some cyanine dyes in a cyanine dye–TiO colloids system

The absorption and fluorescence spectra of five typical cyanine dyes adsorbed on the surface of TiO colloids have been 2 studied. The absorption spectra show that there are two kinds of isomer produced by intramolecular torsional motion of these dyes in their ground states. The fluorescence quantum yields of these dyes may be enhanced or reduced in the presence of TiO colloids. This behavior ca...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Cytometry

سال: 1990

ISSN: 0196-4763,1097-0320

DOI: 10.1002/cyto.990110313